Tagworks Pharmaceuticals
Tagworks Pharmaceuticals is a privately held pharmaceutical SME based in Eindhoven, The Netherlands, focused on the in vivo assembly and actuation of diagnostic and therapeutic antibody conjugates. Founded in 2011, Tagworks is a spin-out of Royal Philips Electronics, and originates from Philips’ molecular imaging expertise. The Company is located at the High Tech Campus in the Center for Imaging Research and Education (CIRE), which is a shared life science facility between Philips, the Technical University Eindhoven, and third parties. The >2000 m2 infrastructure at CIRE, supported by dedicated scientists and technicians, includes organic and protein chemistry, molecular and cell biology, radiochemistry, and imaging labs. In addition, the Company works with several CRO partners. Tagworks has been successful in securing a number of grants, such as a $585k grant from the National Institutes of Health, USA.

Staff ExpertiseDr Robillard has extensive experience with the click reaction (the inverse-electron-demand Diels Alder or inv-DA reaction) that underlies part of this proposal and his group was the first to publish the use of the inv-DA for in vivo radiolabeling and imaging of tagged antibodies. His expertise in chemical synthesis, antibody conjugation, and in vivo chemistry using the inv-DA is critical to the success of the project. Dr Robillard has extensive experience in supervising research and students, at Tagworks as well as at his previous position at Philips Research, and is committed to provide the appropriate support to the students involved in this exciting training network project.

Key facilities and infrastructureThe infrastructure is dedicated for the development of new imaging and therapeutic agents and formulations. The organic chemistry lab has 12 hoods and includes a preparative column chromatography Combiflash Companion apparatus (Teledyne Isco), a Preparative HPLC Agilent 1200 apparatus, and a freezedryer (Salm & Kip). The ML1-3 labs offer a wide scope of molecular and cell biology tools, e.g. UV measurements with Nanodrop, confocal microscopy, PCR, FACS, 4 cell culture laminar flow hoods. The radiochemistry labs allow synthesis of all kinds of SPECT and PET tracers with 4 shielded laminar flow hoods and 4 hot cells for PET chemistry. It further comprises automated synthesis robots for tracers with high activity, 2 Gamma Counters (Wizard), 3 HPLC systems (Agilent) equipped with UV and radioactive detectors (Gabi, Raytest), a gel electrophoresis PhastSystem (GE Healthcare Life Sciences) and a FLA-7000 phosphorimager (FujiFilm). The imaging laboratory is one of the most comprehensive and modern imaging labs of its kind with all imaging modalities available, i.e. intravital optical microscopy (Olympus) microCT (Scanco), preclinical SPECT/CT (NanoSPECT, Bioscan), preclinical PET (Philips), US scanners (2 HDI 5000, Philips), a human 3T MRI scanner (Achieva, Philips) and dedicated high field small animal scanners 7T (Bruker) and 9.4 T (Bruker). Furthermore, several high intensity focused ultrasound transducers are available for drug delivery studies. All imaging modalities are integrated into the radiochemistry lab (level B), which allows multimodal imaging, biodistribution or therapeutic studies using nuclear imaging and labelling techniques. Adjacent to the LSF, a strong material research department is present allowing a comprehensive characterization of e.g. (in)organic constructs, nanoparticles, using e.g. NMR (Bruker DPX300 spectrometer; Bruker Avance600 spectrometer), LCMS (Agilent ESI-TOF mass spectrometer), MALDI, IR, UV, elemental analysis, cryo TEM, TEM, DLS, DSC, x-ray scattering, and many other techniques.

Previous involvement in Research Programmesno

Current Involvement in Research Programmesno

Publications• Versteegen R.M. et al. Angew. Chem. Int. Ed. (2013)
• Rossin R. et al. J. Nucl. Med. (2013)
• Rossin R. et al. Bioconjug. Chem. (2013)
• van den Bosch S.M. et al. Nucl. Med. Biol. (2013)
• Rossin R. et al. Angew. Chem. (2010)

Overview of all participants ...